The present invention relates to generation and high level expression of recombinant nontoxic of epsilon toxin of Clostridium perfringens as a recombinant vaccine against Clostridium perfringens infection and a process for producing the vaccine involving amplifying, cloning, transforming, incubating and purifying the recombinant non-toxic epsilon toxin protein. Thus in this invention, substitution mutation Y7IG was executed in recombinant Etx and the recombinant EtxY7IG protein was over-expressed in soluble form. Expressed protein was purified near homogeneity by DEAF sepharosc anion exchange chromatography with high yield. Potential of rEtxY71G as a vaccine candidate was evaluated and found to be highly specific and immunogenic. The present invention is the first report for high level expression of non toxic rEtxY7IG mutant protein of Clostridium perfringens. Upto 100 mg/L of highly immunogenic and homogeneous recombinant EtxY7IG protein of 31 kl)a was produced. Further, the immunization with rEtxY71G gave very high titer and conferred protection against epsilon toxin intoxication.
