SHIN HEE SOON,신희순,KIM SEUNG YONG,김승용,SHIN DONG UK,신동욱,EOM JI EUN,엄지은,LEE SO YOUNG,이소영,JUNG SUN YOUNG,정선영,CHOI DAE WOON,최대운,NAM YOUNG DO,남영도,PARK SO LIM,박소림
申请号：
KR1020190109803
公开号：
KR1020200027455A
申请日:
2019.09.04
申请国别(地区):
KR
年份:
2020
代理人:
摘要：
The present invention discloses a composition for ameliorating a pulmonary disease such as chronic obstructive pulmonary disease (COPD) or the like by using an extract of Euonymus alatus. A food composition for reinforcing a respiratory function and ameliorating a respiratory disease comprises an extract of Euonymus alatus as an active ingredient. The extract of Euonymus alatus has activities of inhibiting Netosis activation of Neutrophil extracellular traps induced by phorbol myristate acetate (PMA), inhibiting expression of IL-8, i.e., inflammatory cytokines caused by CSE of NCI-H292 cells, i.e., a bronchial epithelial cell line producing a phlegmatic temperament, and inhibiting expression of MIP-2, i.e., inflammatory cytokines of MH-S cells, i.e., alveolar macrophages activated by CES and lipopolysaccharide (LPS). Further, the extract of Euonymus alatus has activities of inhibiting damages to lung tissues in a respiratory disease mouse model induced by CSE and PPE, inhibiting infiltration of immune cells such as total cells, macrophages, lymphocytes, eosinophil, neutrophils, and others within BALF, and inhibiting secretion of inflammatory cytokines and chemokines in BALF and lung tissues.COPYRIGHT KIPO 2020본 발명은 화살나무 추출물을 이용한 만성폐쇄성폐질환 등의 폐질환 개선용 조성물을 개시한다. 화살나무 추출물은 PMA(phorbol myristate acetate)에 의해 유도된 호중구 세포외 트랩(Neutrophil extracellular traps)의 네토시스(Netosis) 활성화를 억제하고, 점액질을 생성하는 기관지 상피세포주인 NCI-H292 세포의 CSE에 의한 염증성 사이토카인인 IL-8의 발현을 억제하며, CES와 LPS(lipopolysaccaride)에 의해 활성된 폐포 대식세포인 MH-S 세포의 염증성 사이토카인인 MIP-2의 발현을 억제하는 활성을 가진다. 또한, CSE와 PPE로 유도된 호흡기 질환 마우스 모델에서 폐 조직 손상을 억제하고, BALF 내에 총 세포(Total cell), 대식세포(macrophages), 림프구(Lymphocytes), 호산구(Eosinophil), 호중구(Neutrophil) 등의 면역세포 침윤을 억제하며, BALF 및 폐 조직에서 염증성 사이토카인과 케모카인 분비를 억제하는 활성을 가진다.
