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SPOSÓB OCZYSZCZANIA PEGYLOWANEJ ERYTROPOETYNY
专利权人:
F.HOFFMANN-LA ROCHE AG
发明人:
FALKENSTEIN, ROBERTO,KOEHNLEIN, WOLFGANG,KUHNE, WOLFGANG,SCHURIG, HARTMUT
申请号:
PL11754693
公开号:
PL2616101T3
申请日:
2011.09.13
申请国别(地区):
PL
年份:
2015
代理人:
摘要:
Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly(ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange SP Sephacryl™ S 500 HR chromatography material conditioned to a conductivity of 21 mS/cm and a linear gradient elution a fusion protein of erythropoietin and a single poly(ethylene glycol) residue can be obtained in a single step with high purity and yield.
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中国工程科技知识中心
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http://www.ckcest.cn/home/

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