An anti-EGFR and anti-CD3 bispecific antibody and its application are provided. The bispecific antibody can specifically bind to epidermal growth factor receptor EGFR, which is a tumor cell surface antigen, and immune cell surface antigen differentiation 簇 3, that is, a CD3 molecule, and the single chain antibody ScFV of anti-CD3 antibody is anti-EGFR. Located at the C-terminus of the antibody constant region. Further provided is a method for preparing this bispecific antibody and its clinical use. The bispecific antibody has high affinity and is used for the treatment of tumor diseases caused by high or abnormal expression of EGFR and other diseases caused by overexpression of GFR.抗ＥＧＦＲと抗ＣＤ３の二重特異性抗体及びその応用を提供する。前記二重特異性抗体は腫瘍細胞表面抗原である表皮増殖因子受体ＥＧＦＲと免疫細胞表面抗原分化簇３、即ちＣＤ３分子に特異的に結合でき、抗ＣＤ３抗体の一本鎖抗体ＳｃＦＶは抗ＥＧＦＲ抗体定常領域のＣ末端に位置する。この二重特異性抗体の調製方法及びその臨床使用をさらに提供する。前記二重特異性抗体は高い親和性を有し、ＥＧＦＲ高発現または異常発現による腫瘍病気、及びその他のＧＦＲ過剰発現による病気の治療に用いられる。
