A light-guiding photobioreactor for culturing microalgae is disclosed. The light-guiding photobioreactor comprises an incubator, a lightening module and a fixed member. The incubator contains a culturing space inside the incubator. The lightening module contains a controlling unit, a light source and at least one light-guiding unit. The controlling unit electrically couples to the light source. The light-guiding unit contains a first end adjacent to the light source and a second end extending into the culturing space. The fixedmember combines the incubator and contains at least one first connector connecting to a floating unit.一種具光導功能之光生物反應器,係用以培養微藻,其包含:一培養箱,內部具有一培養空間;一發光組件,包含一控制元件、一光源及至少一導光單元,該控制元件電性連接該光源,該導光單元具有一第一端及一第二端,該導光單元之該第一端鄰近該光源,且該導光單元之第二端係伸入該培養空間;及一固定件,係結合該培養箱,該固定件具有至少一第一連接部,以連接一漂浮單元。1‧‧‧培養箱11‧‧‧培養槽111‧‧‧培養空間12‧‧‧封閉件12a‧‧‧第一表面12b‧‧‧第二表面121‧‧‧第一穿孔122‧‧‧閥體123‧‧‧導氣管124‧‧‧第二穿孔2‧‧‧發光組件21‧‧‧控制元件22‧‧‧光源23‧‧‧導光單元23a‧‧‧第一端23b‧‧‧第二端3‧‧‧固定件31‧‧‧第一連接部33‧‧‧第二連接部S‧‧‧鎖固元件