2. The production of oligoviruses includes the supply of Clulas per.c6 (ECCC No. 9602940) in a serum-free manner, the infection of these Clulas with oligoviruses and the harvest of the oligoviruses 12-48 hours after infection, and the production of a polio vaccine.
<;p>; PROCESS FOR THE PRODUCTION OF POLIOVIRUSES THAT INCLUDES THE FOLLOWING STEPS: BY PROVIDING A CULTURE OF CEacute; LULAS IN A SUSPENSIOacute; N FREE OF SERUM, WHERE SUCH CEacute; LULAS ARE CEacute; LULAS PER.C6, B) INFECT SUCH CEacute; LULAS WITH POLIOVIRUS, IN A CELL DENSITY BETWEEN 2X106 CEacute; LULAS / MI AND 150X106 CEacute; LULAS / MI, YE) HARVEST THE POLIOVIRUS BETWEEN 12 AND 48 HOURS AFTER THE INFECTION / NUMBER.[Chinese explanation]: ampere, NBS, lt, / P gt<;p>;PROCESO PARA LA PRODUCCIÓ;N DE POLIOVIRUS QUE COMPRENDE LOS SIGUIENTES PASOS: AL PROVEER UN CULTIVO DE CÉ;LULAS EN UNA SUSPENSIÓ;N LIBRE DE SUERO, DONDE DICHAS CÉ;LULAS SON CÉ;LULAS PER.C6, B) INFECTAR DICHAS CÉ;LULAS CON POLIOVIRUS, EN UNA DENSIDAD CELULAR DE ENTRE 2X106 CÉ;LULAS/MI Y 150X106 CÉ;LULAS/MI, Y E) COSECHAR LOS POLIOVIRUS ENTRE 12 Y 48 HORAS DESPUÉ;S DE LA INFECCIÓ;N.<;br />; ;<;/p>;