NATIONAL UNIVERSITY CORPORATION OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE
发明人:
OGAWA, HARUKO,IMAI, KUNITOSHI,TAGAMI, TAKAHIRO
申请号：
EP10820641
公开号：
EP2484757A4
申请日:
2010.09.30
申请国别(地区):
EP
年份:
2013
代理人:
摘要：
A method for producing an α-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme an influenza virus vaccine having a high effect (antigenicity), which is produced using an α-Gal-expressing virus produced by the method and others. Specifically disclosed are: a method for producing an α-galactose epitope (Galα1-3Galβ1-4GlcNAc -R: α-Gal hereinafter)-expressing virus, which comprises the steps of:(1) introducing an α1,3-galactosyltransferase gene in an expressible condition into a cell line that does not express α-Gal to obtain a cell line capable of expressing α-Gal(2) inoculating a virus into the cell line capable of expressing α-Gal to obtain a cell line infected with a virus and(3) culturing the cell line infected with the virus to obtain a virus expressing α-Gal from the culture medium.
