WEYENS, Guy,LEFÈBVRE, Marc,HAIN, Rüdiger,JOHANN, Gerhard
申请号：
ES13819001
公开号：
ES2684994T3
申请日:
2013.12.13
申请国别(地区):
ES
年份:
2018
代理人:
摘要：
A method of producing a mutant sugar beet plant that is resistant to one or more acetohydroxy acid synthase (ALS) inhibitors, comprising the steps of: - obtaining protoplasts from stoma guard cells isolated from a beet plant sugar bowl； - applying to an in vitro culture of said protoplasts a composition comprising one or more ALS inhibitors at a concentration that is lethal for more than 99% of the cells cultured in vitro； and - regenerating sugar beet plants from the surviving cells of these cultured cells in vitro, in which said guard cell protoplasts in the stoma are pre-selected for their ability to regenerate in a sugar beet plant, in which said pre-selected protoplasts have a probability of more than 10% (number of protoplasts in growth: total number of protoplasts in culture) to divide and regenerate in a viable callus, in which calluses obtained by said protoplasts have more than 10% ( number of callus-producing outbreaks: total number of calluses) of capacity to develop outbreaks, and in which said ALS inhibitor (s) is applied or applied to more than 20,000,000 of said protoplasts.Un método para producir una planta de remolacha azucarera mutante que es resistente a uno o más inhibidores de la enzima acetohidroxiácido sintasa (ALS), que comprende las etapas de: - obtener protoplastos a partir de células guardianas en el estoma aisladas de una planta de remolacha azucarera； - aplicar a un cultivo in vitro de dichos protoplastos una composición que comprende uno o más inhibidores de ALS a una concentración que es letal para más del 99% de las células cultivadas in vitro； y - regenerar plantas de remolacha azucarera a partir de las células supervivientes de estas células cultivadas in vitro, en el que dichos protoplastos de células guardianas en el estoma se pre-seleccionan por su capacidad de regenerarse en una planta de remolacha azucarera, en el que dichos protoplastos pre-seleccionados tienen una probabilidad de más de 10% (número de protoplastos en crecimi
