A medical accessory holder (10) is provided for use during processing of medical equipment. The holder (10) comprises a tray portion (11), retaining means (16, 17) associated with the tray portion (11), and securing means (13) provided on the tray portion (11). The retaining means (16, 17) are adapted to engage with a medical accessory (20) such that, once engaged, the medical accessory (20) cannot be disengaged without breaking the medical accessory holder (10), thereby rendering the holder (10) suitable only for single use. The securing means (13) enable the holder (10) to be secured to an article of medical equipment with which the medical accessory (20) is associated, during processing thereof.