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Design of bifunctional Plasmid in Ewing sarcoma
专利权人:
GRADALIS; INC.
发明人:
JOHN J. NEMUNAITIS,DONALD RAO,NEIL SENZER
申请号:
ARP130101079
公开号:
AR091327A1
申请日:
2013.04.03
申请国别(地区):
AR
年份:
2015
代理人:
摘要:
Components and methods for making and using image imprinting, including an expression vector, wherein a promoter and a nucleic acid inserter operatively connected with the promoter input one or more short fork RNAs (rnhc) capable of inhibiting the expression of the white gene sequence. This is the fusion gene of EWS-FLI1, ewsr1-erg, or both, in Ewing's sarcoma, through RNA interference,Among them, one or more rnhc consists of a bifunctional RNA molecule, which activates an RNA induced silencing complex, which is shear dependent and shear independent, in order to reduce the expression level of white gene. Claim 5: a treatment management system, which is characterized by a delivery vehicle of a therapeutic agent and an expression vector including a promoter and a nucleic acid inserted in combination with the promoter in operation,One or more short fork (rnhc) codes can be inserted to inhibit the expression of white gene, that is, EWS and FLI1 genes are combined and / or ewsr1 and ERG genes are combined into a scabies of Ewing by RNA interference, and one or more rnhc contains a dual function RNA molecule. It can activate a RNA induced silencing complex that depends on and is independent of the cathode,This can reduce the expression level of white gene. Claim 18: a method for implanting one or more rnhc into white tissue, which represents the combination of EWS gene and FLI1 gene and / or the combination of ewsr1 gene and ERG gene, and is characterized by the following steps: developing an expression vector, which includes a promoter and a nucleic acid embedded in the operation a. Promoter, which encodes one or more rnhc, can inhibit the expression of white gene, that is, the combination of EWS gene and FLI1 gene and / or the combination of ewsr1 gene and / or ewsr1 gene and / or ewng gene, through RNA level interference;1. The expression vector and treatment vector are combined. In the treatment vector, the treatment vector is a kind of fat body, which is composed of
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