This protein Protein (Crl1F-CRIFA), is a kind of fiber, it combines its insecticide activity with insect pest, and connects with the receiver of mother cell, and the edge of marginalisation. In the active biomass of biology, the protein of radioactive concentration facilitates the test that is at war with other mercury toxin. 2.It is required that 4: a kind of measuring method, to determine core and recipient the contacting between half stomach in a kind of insect of Cyl1FC-1 toxin, including :(a) prepare a kind of gram and subtract, wherein containing a kind of known film, middle edge of the edge at edge in insects is remote (b) in a separate container, the CRY1F3 of (a) increasing quantity increases to the fixed quantity of the pause injection under time conditions and on the membrane suspension plate under depreciation solution for addition. The label of Cr1FYFA is connected with recipient. (d) all radiated signals are all placed on plus on the glass on thin plate.