DR DURAGKAR NANDAKISHORE JEEVANRAO,DR BHUSARI KISHOR PANDURANG,KATOLKAR PARIMAL PRADIPKUMAR
申请号：
IN455/MUM/2012
公开号：
IN2012MU00455A
申请日:
2012.02.20
申请国别(地区):
IN
年份:
2014
代理人:
摘要：
The present invention provides the new process for the isolation of indole alkaloid from the bark of Moringa oleifera. The process comprises drying, grinding, and hot defatting of M. oleifera bark with petroleum ether. This is followed by the successive sequential hot extraction with the solvents viz ethyl acetate, acetone, methanol and hydroalcohol. The solvents are then removed under vacuum preferably at a temperature in the range of 35-40°C. The hydroalcoholic crude extract obtained thereby is then subjected to column chromatography in chronological order of polarity of solvent starting with petroleum ether. The methanolic fraction showing similar pattern in TLC are mixed together and concentrated in vacuum under reduced pressure to get light brown colour new alkaloid. The crystal is washed to free form any adhere impurities. The crystals are dried and subjected to identification by spectrophotometric methods such as UV, IR, NMR and Mass. The isolated, purified and characterized new alkaloid is subjected to MTT Assay and Capsase-3 activity using cancerous cell line shows good anticancer activity.
