Adam E. Cohen,Joel Kralj,Adam D. Douglass,Daniel Hochbaum
申请号：
US14942992
公开号：
US09702874B2
申请日:
2015.11.16
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.
