It is provided a plant infiltration device for infiltrating plants with an inoculum and method of using same, the device comprising a frame having one or more tray racks adapted for receiving at least one tray of plants to be infiltrated； an automated manipulation mechanism mounted to the frame and operable to displace the plant trays within the device； a plurality of inoculum reservoirs mounted to the frame and containing one or more inoculum fluids； an infiltration tank mounted to the frame and within which the plant trays are received when disposed in the infiltration position； and a vacuum generating device in communication with the infiltration tank to apply a negative pressure within the infiltration tank, thereby opening pores of the plant leaves immersed in the inoculum within the infiltration tank and causing the inoculum fluid to infiltrate into the plant leaves.
