Patrick Hoffmann,Silke Mittelstrass,Jens Hennecke,Tobias Raum
申请号：
US11572116
公开号：
US08518403B2
申请日:
2005.07.15
申请国别(地区):
US
年份:
2013
代理人:
摘要：
A composite polypeptide, said composite polypeptide comprising a desired polypeptide and an expression enhancing domain (“EED”), said EED comprising first and second cysteine amino acid residues Cys1 and Cys2, respectively, Cys1 being located closer to the N-terminus of the composite polypeptide molecule than Cys2, wherein Cys1 and Cys2 are separated by a polypeptide linker, said linker—being free of cysteine and proline—defining a length sufficient to allow Cys1 and Cys2 to engage in an intramolecular disulfide bond with one another and—having a flexible polypeptide conformation essentially free of secondary polypeptide structure in aqueous solution, wherein at least one of Cys1 and Cys2 is derivatized with a derivatization moiety.
