A PHARMACEUTICAL COMPOSITION FOR INHIBITING ANGIOGENESIS COMPRISING HSP27 FRAGMENT AND A METHOD OF SCREENING AN ACTIVE MATERIAL FOR INHIBITING ANGIOGENESIS
KOREA INSTITUTE OF RADIOLOGICAL & MEDICAL SCIENCES;한국원자력의학원
发明人:
LEE, YOON JIN,이윤진,LEE, YUN SIL,이윤실,CHOI, SEO HYUN,최서현,JIN, YEUNG BAE,진영배,KIM, KYUNG JUNG,김경중,LEE, HAE JUNE,이해준
申请号：
KR1020110114131
公开号：
KR1020130049083A
申请日:
2011.11.03
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: A pharmaceutical composition containing a HSP27 fragment for suppressing angiogenesis is provided to effectively suppress angiogenesis, and to treat various diseases related to angiogenesis. CONSTITUTION: A pharmaceutical composition for suppressing angiogenesis contains a HSP27 fragment in which a 1-58th N-terminal amino acid sequence of HSP27 is deleted. A pharmaceutical composition for treating cancer contains the HSP27 fragment. The pharmaceutical composition is used for suppressing cancer metastasis. A pharmaceutical composition for suppressing angiogenesis contains a nucleic acid encoding the HSP27 fragment. A method for screening an active material for suppressing angiogenesis comprises: a step of treating a mixture of the HSP27 fragment and VEGF with a sample； a step of measuring binding level of HSP27 or HSP27 fragment and VEGF； and a step of selecting a sample of which binding level is enhanced over a control group. [Reference numerals] (AA) Immuneprecipitation； (BB) Western blot본 발명은 HSP27의 제 1번 내지 58번의 N-말단 아미노산 서열이 결실된 HSP27 단편(Heat Shock Protein 27 fragment) 또는 그 단편을 암호화하는 핵산을 포함하는 혈관신생 억제용 약학 조성물 및 암 치료용 약학 조성물을 제공한다. 또한, 본 발명은 상기 HSP27 단편을 이용하여 in vitro 에서 혈관내피세포의 성장을 억제하는 방법을 제공한다. 또한, 본 발명은 상기 HSP27 단편을 이용하여 혈관신생 억제용 활성물질 또는 암 치료용 활성물질을 스크리닝하는 방법을 제공한다.
