The present invention relates to a crystalline form of 1-(β-d-glucopyranosyl)-4-methyl-3-[5-(4-fluorophenyl)-2-thienylmethyl]benzene. Specifically, it discloses a novel crystalline form of 1-(β-d-glucopyranosyl)-4-methyl-3-[5-(4-fluorophenyl)-2-thienytmethyl]benzene and the preparation method as well as use thereof. The novel crystalline form of 1-(β-d-glucopyranosyl)-4-methyl-3-[5-(4-fluorophenyl)-2-thienylmethyl]benzene provided in the present invention has the advantages such as stable physical and chemical properties, high purity, excellent reproducibility and industrial production suitability.