PROBLEM TO BE SOLVED: To provide an effective method for producing a population of NK cells for cell therapy, which improves amplification efficiency of NK cells in vitro and flexibly increases the signal required for NK cell licensing. SOLUTION: A population of mononuclear cells, which is derived from a plurality of donors and contains NK cells, is prepared, and the prepared population of mononuclear cells is suitable for conditions effective for NK cell-treated proliferation, for example, suitable for proliferation of NK cells. A method for producing a cell population containing NK cells, which comprises incubating in culture with a medium to grow the NK cells. [Selection diagram] None【課題】細胞療法のためのＮＫ細胞の集団を製造するための、ＮＫ細胞の体外での増幅効率を改善し、ＮＫ細胞のライセンシングに必要なシグナルを柔軟に増やす、有効な方法の提供。【解決手段】複数のドナーに由来し、ＮＫ細胞を含む、単核細胞の集団を準備し、準備した単核細胞の集団をＮＫ細胞処理増殖上有効な条件、例えばＮＫ細胞の増殖に適した培地を用いてインキュベート培養し、ＮＫ細胞を増殖させることを含む、ＮＫ細胞を含む細胞集団の製造方法。【選択図】なし
