A surgery navigation system according to the present invention includes: a positioning module including: a transceiver unit, configured to transmit a frequency modulated signal； multiple positioning marks, which are separately disposed on each vertebra of a spine and configured to transmit the positioning mark frequency signal to the transceiver unit after receiving the frequency modulated signal； and a surgical instrument, which transmits the instrument frequency signal to the transceiver unit after receiving the frequency modulated signal； and a processing unit, which obtains a distance between the positioning marks and the transceiver unit through calculation according to a difference between the positioning mark frequency signal and the frequency modulated signal, and obtains space coordinates of the spine through calculation according to the distance； and obtains a distance between the surgical instrument and the transceiver unit through calculation according to a difference between the instrument frequency signal and the frequency modulated signal, obtains space coordinates of the instrument through calculation according to the distance, and performs a surgery navigation operation according to the space coordinates of the spine and the space coordinates of the instrument.
