VIGNA, Elisa,MICHIELI, Paolo,COMOGLIO, Paolo Maria
申请号：
ES14701430
公开号：
ES2674491T3
申请日:
2014.01.07
申请国别(地区):
ES
年份:
2018
代理人:
摘要：
An Antibody fragment comprising a First polypeptide comprising a light chain variable domain and two Constant domains and a Second polypeptide comprising a variable Domain dAnd heavy chain and two Constant Domains, two Domains are Domains where Constant light chain Constant domains and two Constant Domains are Constant heavy chain CH1,Where the Antibody fragment has a prolonged half-life in vivo with respect to a FAB fragment comprising such Light and heavy chain variable domain.Where the first polypeptide comprises a light chain variable domain and two light chain Constant domains of HumanWhere the light chain variable domain is FUSED to the first Constant domain of light chain that merges the second Constant domain of light chain in the direction of N - terminal CTErminal Chimeric light chain, thereby generating a VL - CL and Cl -, where the second polypeptide consists of a heavy chain variable domain of heavy chain Constant domains and two humanWhere the variable domain of heavy chain FUSED to the first Constant domain of Human heavy chain CH1 merges the second Constant heavy chain CH1 Domain in the HumanThe n-terminus to c-terminus, thereby creating a Chimeric heavy chain VH CH1 - CH1.Un fragmento de anticuerpo que comprende un primer polipéptido que comprende un dominio variable de cadena ligera y dos dominios constantes y un segundo polipéptido que comprende un dominio variable de cadena pesada y dos dominios constantes, en donde dos dominios constantes son dominios constantes de cadena ligera y dos dominios constantes son dominios constantes de cadena pesada CH1, en donde dicho fragmento de anticuerpo tiene la semivida in vivo prolongada con respecto a una fragmento de Fab que comprende dichos dominios variables de cadena ligera y pesada, en donde el primer polipéptido comprende un dominio variable de cadena ligera y dos dominios constantes de cadena ligera humana, en donde el dominio variable de cadena ligera se fusiona al primer dominio constante de cadena
