The present invention provides a lipase inhibitor which includes a limonene. The limonene can effectively inhibit the activity of lipase resulting in blocking of the absorption of lipid from food consumed. Moreover, the present invention also provides a method for manufacture of weight-reducing composition with limonene. The lipase inhibitor and application thereof according to the present invention can thus be utilized as pharmaceutical composition or health supplement for ameliorating obesity, lipid metabolic disorder, or other related syndromes.本發明係提供一種脂肪酶活性的抑制劑,係包含一檸檬烯;該抑制劑中的檸檬烯可有效抑制脂肪酶的活性,進一步阻斷體內吸收來自於飲食之脂肪。另一方面,本發明亦提供一種檸檬烯用於製備減重之組合物的用途。本發明之脂肪酶活性的抑制劑及製備減重之組合物的用途可提供作為一種改善肥胖、脂肪代謝不良、及其引發症狀之醫藥組成物或保健食品。