David Shering;Penny Johnson;Damian Marshall;Paul Kemp;Andrew Shering, legal representative
发明人:
Paul Kemp,David Shering,Penny Johnson,Damian Marshall
申请号：
US11886113
公开号：
US08114670B2
申请日:
2006.03.14
申请国别(地区):
US
年份:
2012
代理人:
摘要：
Methods of forming soft connective tissue compositions such as skin equivalents, compositions made by the methods and their uses. In particular, a method of forming a connective tissue equivalent, comprising the steps of: (i) incubating collagen-producing cells in or on a support matrix； (ii) inducing and/or enhancing collagen production by the collagen-producing cells to form a collagenous construct and degradation and replacement of the support matrix； (iii) freeze-drying the construct； and (iv) re-populating the freeze-dried construct with collagen-producing cells and/or epithelial cells and/or endothelial cells and/or mesenchymal cells, thereby forming a connective tissue equivalent, wherein: (a) the collagen-producing cells are substantially fibroblasts； for example human neonatal dermal fibroblasts； (b) the support matrix is a provisional support matrix in which the support matrix is a fibrin matrix, for example formed by thrombin-mediated polymerisation of fibrinogen； and (c) as a result of the collagen production by the collagen-producing cells the provisional fibrin support matrix is digested by the cells and is replaced by collagen, thereby essentially replacing the provisional fibrin matrix with a collagen matrix synthesised in situ by the cells.
