An isolator (1) includes a workroom (10) having a first sterilizing material supply port (16), a first sterilizing material discharge port (110), a first gas supply port (18) and a first gas discharge port (20), a sterilization chamber (40) having a second sterilizing material supply port (42), a second sterilizing material discharge port (112), a second gas supply port (44) and a second gas discharge port (46), a sterilizing material supply unit (200), a first sterilizing material supply path (60) connecting the sterilizing material supply unit (200) to the first sterilizing material supply port (16), a second sterilizing material supply path (62) connecting the sterilizing material supply unit (200) to the second sterilizing material supply port (42), a first circulating path (114) connecting the first sterilizing material discharge port (110) to the sterilizing material supply unit (200), a second circulating path (116) connecting the second sterilizing material discharge port (112) to the sterilizing material supply unit (200), a first gas supply path (64), a second gas supply path (66), a first gas discharge path (68), a second gas discharge path (70), and a control unit (300).
