Michael Gallo,Jaspal Singh Kang,Craig Robin Pigott,Abby Lin
申请号：
US14384772
公开号：
US09718871B2
申请日:
2013.03.14
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Methods of generating sequence diversity in a protein, such as a ligand-binding protein, are provided. The methods comprise targeted introduction of two or more recombination signal sequences (RSSs) into the protein coding sequence and introduction of the modified protein coding sequence into a recombination-competent host cell, specifically a recombination-competent host cell that is capable of expressing at least RAG-1 and RAG-2, thereby allowing for recombination of the protein coding sequence and expression of variant proteins. Also provided are polynucleotides comprising a nucleic acid sequence encoding a target protein, such as a ligand-binding protein, and comprising two or more RSSs, and compositions and host cells comprising same.
