Provided are a method and device for locating a human target using superficial vein characteristics. According to characteristics that a position of the human target is fixed relative to superficial veins and that the superficial veins are easy to be observed by a vein display device, a locating model for displaying both of the superficial veins and the human target is generated to achieve accurate locating of the human target. The device has the advantages of a simple structure, convenience in use, easiness in operation and a high accuracy; the device may be used at any angle and within a wide range; the device may locate the human target at any angle and position due to small interference from the surrounding environment; and the device is convenient to install and disassemble, an appropriate mold or projection manner may be selected according to different human targets. Accurate locating is achieved in clinical use, and time and efforts of a doctor to repeatedly explore the human target according to experience are reduced.