An assembly tool for use with an ultrasonic surgical instrument includes a body portion, a neck, and a head portion. The body portion includes a base and first and second legs extending from the base to define a receiving area therebetween. The neck is attached to the body portion and the head portion is attached to the neck. The head portion defines a central opening. The first and second legs of the body portion are configured to engage a portion of a housing of an ultrasonic surgical instrument received within the receiving area, and the head portion is configured to engage a rotation knob of the ultrasonic surgical instrument received within the central opening to rotationally fix the rotation knob relative to the housing. Kits including the assembly tool and methods of assembling an ultrasonic surgical instrument using the assembly tool are also provided.
