Abstract METHOD OF BIOLOGICAL SYNTHESIS OF ZINC OXIDE (ZnO) NANOPARTICLES This present invention related to method for biological synthesis of zinc oxide (ZnO) nanoparticles using a extract of shoots of Azadirachta indica, shoots of Ficus religiosa and pedicels of Catharanthus roseus reports a biological benevolent route of Zink Oxide (ZnO) nanoparticles (NPs) synthesis using the extract of Catharanthus roseus (L.) G. Don (C.roseus), Azadirachta indica (A. indica) and Ficus religiosa (F.religiosa) involving phenomenon of temperature gradient by varying reaction temperature and NaOH concentration respectively. The anti- cancer activity of ZnO NPs was investigated against human carcinoma cells viz SCC-40, SK-MEL-2 and SCC-29B using Sulforhodamine-B (SRB) Assay. The results reveal that the cancer-cell selectivity of the ZnO samples was size dependent as different samples showed better activity to their corresponding cancer cell lines. These nanoparticles were found to be the most active anti- cancer agent prepared using plants™ extract, which causes >50% inhibition of SCC-40, SK-MEL-2 and SCC-29B cell growth at concentrations <80 µg/ml and more than 50% cell kill for SCC-29B cells. The combination of these plants may prove to be an effective anti-cancer agent with potential application in cancer treatment. For 1. KOUTU, VAIBHAV 2. MALIK, MOHAMMAD MANZAR Balram Singh Yadav Regd. Patent Agent [IN/PA-2661] Dated: 07 September 2017
