The process for extraction of lipoaspirate from adipose tissue using by cannula, lipoaspirate is used to isolate mesenchymal stem cells. Autologous mesenchymal stem cells isolated from extracted lipoaspirate are intramuscularly applied and differentiated into ICA�s on suitable medium and in vivo transplanted into liver to use as regenerative medicine for therapeutic benefit of diabetes patient. The process involves aspiration of 400-600cc from adipose tissue is by 4mm multi hole cannula. Lipoaspirate is treated with PBS (Phosphate-Buffered Saline) and optionally supplemented with antibiotics. Lipoaspirate is treated with Collagenase- I. Lipoaspirate is treated to isolate 200-250 million mesenchymal cells through multiple centrifugations. The isolated mesenchymal cells are applied intramuscularly, 1cc in each of deltoids and gluteus maximus muscles with 21no. Needle in proportion from 1.0-2.0�104-1.0-2.0� 108 cells/kg of body weight using Isolyte-M as carrier for application. The mesenchymal cells are cultured on DMEM medium to obtain ICA�s. ICA�s are confirmed by Dithizone (DTZ) stain. ICA�s are used for in vivo transplantation in liver in the proportion of 1.0-2.0�102-1.0-2.0�104 ICA/kg of body weight by a surgeon using 21 no. needles.
