The invention concerns a single-use dental floss holder that includes a base portion, first and second spaced-apart arms integral with and extending from the base portion, each spaced-apart arm having a distal section, and a length of dental floss secured between the distal sections of the spaced-apart arms, where the length of dental floss include applied thereto about 10 percent by weight or more of a wax composition, based on the weight of the length of dental floss and to methods of making the single-use dental floss holder, the method including the steps of providing a mold having a cavity for receiving molten plastic and a groove having an aspect ratio of about 10:1 or greater for receiving a length of wax coated dental floss, positioning the length of dental floss in the groove of the mold, injecting the cavity of the mold with the molten plastic and cooling the molten plastic about the length of dental floss having the wax composition applied thereto.