The Governing Council of the University of Toronto
发明人:
Andrew Emili,Megan McLaughlin,Kyrylo Zagorovsky,Jonathan Buchanan Olsen,Warren C.W. Chan,Sachdev S. Sidhu
申请号：
US16686549
公开号：
US20200141944A1
申请日:
2019.11.18
申请国别(地区):
US
年份:
2020
代理人:
摘要：
The invention describes methods and reagents useful for sequencing polypeptide molecules. The method comprises affixing a polypeptide to a substrate and contacting the polypeptide with a plurality of probes. Each probe selectively binds to an N-terminal amino acid or an N-terminal amino acid derivative. Probes bound to the polypeptide molecule are then identified before cleaving the N-terminal amino acid or N-terminal amino acid derivative of the polypeptide. Also provided are methods for the sequencing a plurality of polypeptide molecules in a sample and probes specific for N-terminal amino acids or N-terminal amino acid derivatives.
