Disclosed herein are non natural amino acids and dolastatin analogs that include at least one non natural amino acid and methods for making such non natural amino acids and polypeptides. The dolastatin analogs can include a wide range of possible functionalities but typically have at least one oxime carbonyl dicarbonyl and/or hydroxylamine group. Also disclosed herein are non natural amino acid dolastatin analogs that are further modified post translationally methods for effecting such modifications and methods for purifying such dolastatin analogs. Typically the modified dolastatin analogs include at least one oxime carbonyl dicarbonyl and/or hydroxylamine group. Further disclosed are methods for using such non natural amino acid dolastatin analogs and modified non natural amino acid dolastatin analogs including therapeutic diagnostic and other biotechnology use.
