PROBLEM TO BE SOLVED: To remove an erythrocyte from inside a cell originating from a living tissue, which is obtained from a cell suspension by concentration separation, and thus to perform the concentration separation of the cell originating from the living tissue which has high concentration.SOLUTION: A centrifuging container 1 provided herein includes a tubular container body 2 which has a bottom 2a closed in a tapering shape at one end and an inlet 5 of the cell suspension A at the other end, a first partition wall 3 which partitions the inside of the container body 2 into a bottom side space 7 and an inlet side space 8 in the longitudinal direction, and a second partition wall 4 which partitions the bottom side space 7 into a first space 11 being along the inside surface of the container body 2 and a second space 12 being inside the first space 11. The first partition wall 3 is provided with a main channel 9 of a caliber making the inlet side space 8 and the second space 12 communicate with each other and making the flowing cell suspension A produce a Fahraeus-Lindqvist effect, and with branch channels 10a and 10b making a flow near a wall surface branch at a middle position of the main channel 9 and making it communicate with the first space 11.COPYRIGHT: (C)2012,JPO&INPIT【課題】細胞懸濁液から濃縮分離される生体組織由来細胞内から赤血球を取り除き、濃度の高い生体組織由来細胞を濃縮分離する。【解決手段】一端に先細に閉塞された底部2aを有し、他端に細胞懸濁液Aの導入口5を有する筒状の容器本体2と、該容器本体2内を長手方向に底部側空間7と導入口側空間8とに区画する第1の区画壁3と、底部側空間7を容器本体2の内面に沿う第1空間11と、該第1空間11よりも内側の第2空間12とに区画する第2の区画壁4とを備え、第1の区画壁3に、導入口側空間8と第2空間12とを連絡し、流動する細胞懸濁液Aにファーレウス・リンドクイスト効果を発生させる口径の主流路9と、該主流路9の途中位置において壁面近傍の流れを分岐して、第1空間11に連絡する分岐流路10a,10bとが設けられている遠心分離容器1を提供する。【選択図】図1
