The present invention relates to fluid flow diversion in medical procedures, and, more particularly, to a valve assembly for preventing retrograde flow of urine from a collection device into a urinary catheter so as to reduce the risk of and/or prevent catheter-associated urinary tract infection (CAUTI). The valve assembly of the present invention is a discrete, stand-alone valve assembly configured to provide a fluid connection between a urinary catheter (e.g., Foley catheter), and a collection device (e.g., urine drainage bag) or the tubing for coupling the catheter to the collection device. The valve assembly is configured to permit antegrade flow of urine from the catheter into the collection device, while preventing retrograde flow of urine (e.g., backflow) from the collection device into the catheter, thereby reducing the risk of CAUTI.