A sterilization method characterized in that the sterilization method includes carrying out sterilization including allowing an object to be sterilized to stand within a container to which a reactive oxygen is previously irradiated; for example, a sterilization method characterized in that the sterilization method includes irradiating an object to be sterilized comprising a dent portion with a reactive oxygen, stacking an object to be sterilized on top thereof to cover the dent portion, and carrying out sterilization within a formed closed space. The sterilization method of the present invention shows excellent sterilization activity, so that it can be suitably used in, for example, sterilization of containers for foodstuff, bottle caps sealing the openings of the containers, medical devices, foodstuff such as vegetables and meat, and the like.