Described herein is the method of preparation for amnion based tissue conduits. Amnion based tissue conduits are obtained from placental and umbilical cord tissue. Wherein said tissues are separated into one or more layers of amnion, chorion and umbilical cord and incised into predetermined measurements. By incising tissues, the quantified measurements will be less readily able to degrade bioactive properties during the predetermined duration of exposure of super critical carbon dioxide sterilization and a disinfectant wash of amnion, chorion and umbilical cord.
