Disclosed in the present invention is a method for preparing an oil-dispersible carotenoid preparation, comprising (by weight parts): mixing 100 parts carotenoid microcapsule, 100-400 parts vegetable oil and 0.1-1 part oil-soluble antioxidant; and grinding the mixture in a colloid mill in a nitrogen atmosphere and at 10-30°C to obtain a uniform oil-dispersible carotenoid preparation, wherein the preparation contains 2%-14% carotenoid with an average particle size of up to 0.1-1 µm, and 100 parts carotenoid microcapsule contains 10.5-35.8 parts carotenoid, 0.1-1 part water-soluble antioxidant, and the remainder is a water-soluble colloid. The advantages of the present invention lie in that the oil-dispersible form has a high stability as the surface of the carotenoid particles is still protected with a dense water-soluble colloid, and that an oil-dispersible carotenoid preparation containing carotenoid with a content of up to 2%-14% and an average particle size of only 0.1-1 µm can be prepared.