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RECOMBINANT ANTIBODIES TO SCLEROTINIA ANTIGENS
专利权人:
THE GOVERNORS OF THE UNIVERSITY OF ALBERTA
发明人:
KAV NATARAJ,YAJIMA WILLIAM,YANG BO
申请号:
US20070829513
公开号:
US2008104734(A1)
申请日:
2007.07.27
申请国别(地区):
美国
年份:
2008
代理人:
摘要:
The invention is directed to recombinant antibodies which bind to Sclerotinia sclerotiorum antigens and comprise a single chain variable fragment (scFv). The antigen may be selected from SSPG1d or a portion thereof, aspartyl protease or a portion thereof, or whole Sclerotinia sclerotiorum mycelium. The invention also provides an antibody linked to an anti-fungal polypeptide. The invention extends to nucleic acid sequences encoding the antibodies, and expression vectors comprising the nucleic acid sequences. The invention is also directed to transgenic plants, seeds, tissues or cells transformed with the expression vectors. Methods for producing a transgenic plant that is resistant to Sclerotinia sclerotiorum, and for detecting Sclerotinia sclerotiorum in a biological sample utilizing an antibody which binds to Sclerotinia sclerotiorum antigen, and immunoassay kit for same are also provided.
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