The present invention relates to a stable antiseptic solution comprising: at least one di(4-chloro-phenyldiguanido) derivative or a pharmaceutically acceptable salt thereof, wherein said di(4-chloro-phenyldiguanido) derivative or a pharmaceutically acceptable salt thereof is a compound of formula (I) or a pharmaceutically acceptable salt thereofand an at least one anionic dye, wherein the anionic dye or the total amount of anionic dyes present in the antiseptic solution are in an amount sufficient to stain a patients skin or surgical field before surgery, when applied wherein the antiseptic solution is essentially free of any cationic excipient.
