A toothbrush with the function of detecting plaque includes a hand-held portion and an operating portion longitudinally and detachably mounted to the hand-held portion, wherein the hand-held portion has a power set disposed therein and a switch mounted thereon and electrically connected to the power set. A brush head and a light source are respectively disposed on a free end of the operating portion and electrically connected to the power set and the switch. Before brushing teeth, the user turns on the switch and the light source illuminates ultraviolet and the teeth are lightened. As a result, the plaque on the teeth will cause a fluorescent reaction such that the user can check his/her teeth on a mirror and enhances clean action for the teeth that has a fluorescent reaction formed thereon.一種具有牙菌斑檢測功能之牙刷,其主要係具有一握持部以及一軸向連設於握持部之作動部;其中該握持部中係設有一電源組以及一電性連接至電源組之開關;而該作動部異於握持部之一端則裝設有無數刷毛並裝設有一發出紫外線(具315-400μm波長之UVA)之光源,該光源係電性連接至該開關與該電源組。使用者於刷牙前可先打開開關令光源發出紫外線並直接照射於牙齒上,令牙齒長有牙菌斑之部位產生螢光現象,再利用手持之鏡子或浴室中鏡子觀察自己的牙齒,針對發出螢光之部位加強清潔動作,以維護牙齒的健康。10‧‧‧握持部11‧‧‧電源組12‧‧‧開關13‧‧‧光源20‧‧‧作動部21‧‧‧刷頭211‧‧‧刷毛25‧‧‧導光條