A three-dimensional thread making method using ultrasonic waves is provided. The method includes inserting a yarn into a position corresponding to an engraved pattern of a mold base between an ultrasonic wave generator and the mold base at positions adjacent to each other, applying ultrasonic waves to the yarn while the ultrasonic wave generator pressurizes the yarn, and injecting a medical anti-loosening member made in a form of the engraved pattern due to the ultrasonic waves. The medical anti-loosening member includes a medical three-dimensional thread formed to have a plurality of protrusions facing each other on both sides and a medical anti-loosening screw.
