Pascal Charles Louis GERKENS,Michele Therese Rita LECOCQ,Tatsuya KASUGAYA,Kenjiro KAWATSU,Yoshinobu MIYATSU,Tetsuro TANABE
申请号：
US14914341
公开号：
US20160208220A1
申请日:
2014.08.28
申请国别(地区):
US
年份:
2016
代理人:
摘要：
A method of producing a virus in cell culture comprising at least the steps of a) providing a population of cells cultured in a cell culture medium, b) infecting the population of cells by i. inoculating the population with the virus, and ii. incubating the inoculated population so as to allow the virus to replicate and propagate, c) collecting the produced virus, thereby providing a viral harvest, and d) purifying the virus, wherein a power density of at least 15 W/m3, at least 30 W/m3, at least 60 W/m3, at least 100 W/m3, at least 120 W/m3 is applied to the cell culture at least during step b).
