A method for characterizing ultrasound scatterers in a medium comprising providing ultrasound data representing a region of interest comprising a plurality of scatterers in a medium, the plurality of scatterers including clusters of scatterer sub-units, the scatterers having a physical property value to be estimated and the scatterer sub-units having at least one known physical parameter value； modelling the ultrasound data using an at least second order function of a spatial organization parameter defining the spatial organization of the scatterers； and estimating the physical property value of the scatterers from the modelled ultrasound data and the at least one known physical parameter of the sub-units by a regression of the spatial organization parameter as a function of frequency. A system for characterizing ultrasound scatterers is also included.
