A NOVEL INSECTICIDAL COMPOSITION AND METHOD OF PREPARATION THEREOF ABSTRACT The present invention relates to a novel insecticidal formulation and method of preparation thereof. More particularly this invention relates to a composition or formulation comprising Chitinase, Tris Buffer, Neutral Detergents and Protein in a solvent stabilizer and/or an aerosol form with permitted excipients and preservatives useful in killing mosquitoes and other insects.