Method generates and the protein immunization checkpoint (ICPs) of the antibody glycosylation of screening specific bond is provided relative to non-glycosylated ICPs. It the glycosylation ICPs of this antibody identification defined epitope and prevents or blocks in conjunction with glycosylation ICP and its ligand, such as another ICP, and can inhibit interaction between two kinds of albumen will lead to immunosupress, such as interacted by human PD-L 1/PD-1. As a specific example, human PD-L 1 and PD-1 include glycosylated polypeptide its extracellular domain amino acid residue provide for generate resist glycosylated PD-L1 or anti-PD-1 antibody glycosylations specifically combine PD-L1 or PD-1, and inhibit PD-L1/PD-1 interactions. The antibody of generation and selection are interacted wherein by destruction of the method especially suitable for treatment of cancer, prevention or neutralization ICP system and specific ICP.
