A sterilization tray is disclosed herein. A medical instrument, such as an endoscope, may be placed into the sterilization tray, and the sterilization tray with the medical instrument disposed therein may be placed into a vacuum chamber of a sterilization system. The sterilization tray may also be used to transport the medical instrument to and from a point of care. Sterilization is performed with the instrument in the base component and the lid component disposed atop the base component. Transportation is performed with the instrument in the lid component and the base component disposed atop the lid component.