A feed material composition manufacturing method for horned frogs includes: providing a crude feed material adding a predetermined percentage of probiotic to form a formula feed material processing the formula feed material to form a formula feed product which is tastable. The probiotic can improve intestinal micorflora, constipation and excrement smell, increase immunity and further reduce a death rate of horned frogs.一種角蛙配方飼料組成物製造方法包含:提供一角蛙飼料原料;將一益生菌添加於該角蛙飼料原料,以形成一角蛙配方飼料,且該益生菌具有一預定百分比;及將該角蛙配方飼料加工製成一角蛙配方飼料產品。該益生菌具有適口性,以改善腸道消化、改變腸道菌項、減少便秘情形、降低糞便異味、提升免疫力及降低死亡率。1‧‧‧角蛙飼料原料2‧‧‧益生菌3‧‧‧角蛙配方飼料產品100‧‧‧加工設備