Systems and methods for determining microvascular functions in a sample of a subject are provided. A system obtains one or more spectral interference signals from the sample during one or more scans, extracts data from the spectral interference signals concerning cell, tissue, or particle motion within the sample via one or more optical microangiography algorithms, and calculates volumetric properties from the data indicative of fluid motion within the sample. The system and method may be used for diagnosing, providing a prognosis, or monitoring treatment of a disorder of the sample.
