Device for the collection of biological samples in order to obtain the replication of bacteria contained in a biological sample to detect the presence of said bacteria, comprising at least a test tube (13) able to contain the sample taken and into which a culture or growth broth (32) is introduced in order to grow bacteria, and a pick-up unit (12) to take the biological sample, able to be inserted partly inside the test tube (13), wherein the pick-up unit (12) comprises a first stopper (14) to close the test tube (13), a tubular suction member (18) associated with one side of the first stopper (14) in order to take in the culture or growth broth (32), a hollow rod (15) associated with an opposite side of the first stopper (14) and able to be inserted into the test tube (13). The hollow rod (15) is connected fluidically at one end to the tubular suction member (18) and at the opposite end provided with at least a collection swab (17, 1 17) for the biological sample, able to be housed inside the test tube (13), the collection swab (17, 1 17) including passage ways able to put the inside of the test tube (13) in communication with the inside of the hollow rod (15), so as to allow the culture or growth broth (32) taken in to flow through the hollow rod (15), from the tubular suction member (18) toward the inside of the test tube (13).
