Bragg Robert Richard,Boucher Charlotte Enastacia,Theron Chrispian William,Hitzeroth Arina Corli
申请号：
US201615266632
公开号：
US9963487(B2)
申请日:
2016.09.15
申请国别(地区):
美国
年份:
2018
代理人:
Salwanchik, Lloyd & Eisenschenk
摘要：
The present invention relates to the expression of polypeptides using Yarrowia lipolytica, in particular the secretion of expressed polypeptides into either the extracellular space or the surface of the Y. lipolytica host cell wall. The invention also extends to the use of the polypeptides so expressed in biotechnological applications. The present invention provides an expression construct for the expression of polypeptides using at least a single Yarrowia lipolytica yeast cell, the expression construct having at least one expression cassette, the expression cassette including an acid extracellular protease secretion signal sequence and flanking zeta sequence recombination sites.
