The method of including useful low-molecular hairpin RNA (shRNA) for obstructing the gene revelation such as virus mediation characteristic gene revelation, the composition, and the kit are stated.ウイルス媒介性遺伝子発現などの遺伝子発現の阻害のために有用な低分子ヘアピンRNA(shRNA)を含む方法、組成物、およびキットが記載される。